Re: [Histonet] Malt diastase
I use Sigma porcine pancreatic amylase, 0.25% in 4mM sodium acetate
buffer pH 6.0, for 10-15 minutes at room temp. This works well on liver
fixed in buffered formalin.
Geoff
Deborah Faichney wrote:
>Dear all,
>
>I need to obtain malt diastase for Periodic acid schiff staining for
>glycogen in blood smears. I am finding this difficult (in the UK) and
>would like to know from any carbohydate people out there what I could
>use instead.
>
>Thanks
>
>Debbie Faichney
>Institute of Aquaculture
>Stirling, Scotland
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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