Re: [Histonet] Malt diastase

From:Geoff McAuliffe

I use Sigma porcine pancreatic amylase, 0.25% in 4mM sodium acetate 
buffer pH 6.0, for 10-15 minutes at room temp. This works well on liver 
fixed in buffered formalin.


Deborah Faichney wrote:

>Dear all,
>I need to obtain malt diastase for Periodic acid schiff staining for
>glycogen in blood smears.  I am finding this difficult (in the UK) and
>would like to know from any carbohydate people out there what I could
>use instead.
>Debbie Faichney
>Institute of Aquaculture
>Stirling, Scotland

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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