-30 is too cold to section testicles.  I cut them at -15 C. Sometimes even -12 C.

> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of Inga Hansson
> Sent: 	Thursday, January 25, 2007 4:54 AM
> To: 	histonet@lists.utsouthwestern.edu
> Subject: 	[Histonet] testicles
> 
> Hi everyone,
> 
> A collegue of mine is trying to section frozen rat testicle (perfused 
> and cryoprotected in sucrose) at -30°C. Is that a good temperature to 
> use? The sections seem fragile and tend to break. They´re 10 microns 
> thick. Or is it bad fixation?
> 
> Thanks in advance for any help!
> 
> Inga
> 
> 
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