RE: [Histonet] Sudan IV staining

From:"Liz Chlipala"


We routinely perform this on mouse aortas, it quite labor intensive and
requires a person that has the patience and good attention to detail to do
the job correctly.  It all starts from correctly dissecting out the aorta
from the animal initially, but I would guess that rat aortas would be easier
than a mouse and we routinely perform this on mouse aortas.  I can send the
person a powerpoint presentation that might help.  I do not have time to
help this week, but possibly the middle of next week they could contact me
and I could forward the information that I have on the technique.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of Jennifer
Sent: Thursday, January 18, 2007 4:25 PM
Subject: [Histonet] Sudan IV staining

A researcher at an area hospital has asked for some help performing Sudan 
IV staining of rat aortas.  She would like to look for atherosclerotic 
lesions by en face analysis.  I am not familiar with this.  I have 
reviewed the paper she is using as a reference and it does not go into 
detail about the staining protocol for this.  The journal article is 
entitled " Synthetic LXR ligand inhibits the development of 
atherosclerosis in mice 
Is there anyone on the histonet who performs en face analysis and can 

Thank you,
Jennifer MacDonald
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