RE: [Histonet] Stains for Lipids

From:"Monfils, Paul"

The same staining procedures that are used for frozen sections could be used on the paraffin sections, but normally one would not expect to see any lipids in a paraffin section since the tissue has gone through several changes of lipid solvents enroute to paraffin. Yes, tissues fixed in formalin or formol saline can be frozen sectioned and yield excellent lipid stains. Formalin is a good lipid fixative.

> ----------
> From: on behalf of Tamara Melville
> Sent: 	Tuesday, January 23, 2007 6:30 PM
> To:
> Subject: 	[Histonet] Stains for Lipids
> Hello,
>   I would like to stain sections of rat aorta and heart for lipids. I am aware that frozen sections are required however in some paraffin sections stained with H&E I saw some structures resembling fat droplets and would like confirmation by using a stain for lipids. I would like to know if there are any methods to stain lipids in paraffin sections.
>   I would also like to know if tissues fixed and preserved in formal saline can be stained with any of the special lipid stains or are these stains specifically for frozen tissues.
>   Thanks in advance for any assistance.
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