RE: [Histonet] HER2 and the 48 hour rule

From:"patsy ruegg"

If you truly fixed in formalin for 48hrs. wouldn't it be SAFE to leave the
sample in 70% alcohol until processing, adequate formalin fixation will
protect the sample from alcohol damage to the her2 protein.

-----Original Message-----
[] On Behalf Of Rene J Buesa
Sent: Tuesday, January 23, 2007 1:43 PM
To: Douglas D Deltour; 'sheila adey';;;
Subject: RE: [Histonet] HER2 and the 48 hour rule

When you start commercial production, please send me the price per gallon!
  René J.

Douglas D Deltour  wrote:
  I have just patented (in my mind) the idea of "HER2-Safe". It is a holding
liquid that you can load in your processor. It will be pumped into your
retort after the 48 hours of formalin fixation. It will sit in this safe
liquid until you are ready to begin normal tissue processing. This liquid
will not harm your tissue and it is within CAP guidelines for HER2 testing.
No need for your techs to come in over the weekend! Can I get a witness! 

I am opening up bidding for rights to this idea.

Douglas D. Deltour HT(ASCP)
Histology Supervisor
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Fax (803)254-3262

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-----Original Message-----
[] On Behalf Of sheila adey
Sent: Tuesday, January 23, 2007 2:36 PM
Subject: Re: [Histonet] HER2 and the 48 hour rule

This prompts a new question.
I'm curious as to whether other labs that don't work weekends leave their 
tissues in formalin with a delayed start or do you start the processor right

away and leave the tissues in Xylene as stated below?

Sheila Adey HT MLT
Port Huron Hospital

>From: Patti Loykasek 

>To: "Peterson, Dan" <>,histonet 
>Subject: Re: [Histonet] HER2 and the 48 hour rule
>Date: Tue, 23 Jan 2007 10:06:08 -0800
>Dear Dan,
>Welcome to the new world of Her2 testing! There will definitely be changes
>in the lab as a result of the ASCO/CAP Her2 testing guidelines. I urge all
>techs to get a copy of the guidelines and read them.
>That being said, the time in fixation is important for Her2 testing. With
>heat retrieval I think that too little fixation is worse than >48 hours, 
>for now we must comply with 6-48 hours. I was taught (a long time ago) that
>it is best practice for the weekend schedule fixation to reflect the rest 
>the week, and that it is preferable to leave the tissue longer in xylene.
>That xylene would have less bad effects than longer time in formalin. A
>longer time in alcohol would be deleterious to many IHC stains, and not
>recommended for Her2.
>Whatever schedule changes you decide to implement, hopefully you can test
>your new schedule on some tissue before full implementation of a new
>processing schedule.
>Patti Loykasek BS, HTL, QIHC
>PhenoPath Laboratories
>Seattle, WA
> > Hello Histonetters!
> > Maybe this has been discussed before, and if it has, I apologize in
> > advance, but I need a little input. Now that the CAP has mandated a 6
> > -48 hour window of fixation time for specimens that may have Her-2 neu
> > performed, what are you doing about weekend specimens? We JUST (after
> > 25+ years) got rid of the need for techs to come in on Saturdays, and
> > would like to be able to continue this trend. However if a breast bx is
> > done at an outside account on a Thursday afternoon, and does not get
> > grossed by our staff until Friday, right now a our processors are set to
> > start up Sunday afternoon for Monday morning (Kind of over 48 hrs). So
> > here are my questions:
> > Do you set up the processor to sit in 70% OH after the formalin fix? Do
> > you let it sit in the paraffin from Saturday until Monday? (Is heat too
> > prolonged?)
> > Or do I have to break the news to staff that if their name is up, and a
> > breast bx comes in, they're coming in Saturday am?
> > We do not have a microwave processor (yet), but soon.
> > Any and all responses will be greatly appreciated!
> >
> > Daniel R Peterson HT(ASCP)
> > Histopathology Section Head
> > Meriter Laboratories
> > (608)-267-6557
> >
> >
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