RE: [Histonet] H.Pylori

From:"Lee & Peggy Wenk"

I talked with someone in our Microbiology department about this a year or
two ago.

Basically, the answer is - H. pylori cannot be grown in a routine hospital
microbiology lab.

Think of the conditions under which it grows in the stomach - acidic
environment, mucin around it, very low oxygen, plus it is giving off lots of
unusual compounds, such as urease. Routine labs are not set up for this.

As a result, Hp are VERY difficult and trick to grow/culture. If given the
right environment (extremely difficult conditions), Hp will tend to survive
but do not reproduce. The colonies go not get bigger.

So, infortunately, histology and microbiology cannot collaborate to make Hp
controls blocks like we can make other bacteria control blocks.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
[] On Behalf Of Vacca
Sent: Thursday, January 25, 2007 1:05 PM
Subject: [Histonet] H.Pylori

Does anyone that works in a Hospital have their Micro department grow H.
pylori and create their own controls by injecting into the tissue? If you
do, do you mind getting me their procedure for this? Thanks in advance for
your help.

Jessica Vacca
Histology Supervisor
Brandon Regional Hospital
119 Oakfield Dr.
Brandon Fl 33511
(813) 571-5193 or (813) 681-5551 ext 2454

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