RE: [Histonet] Frozen Section H&E's

From:"Terri Braud"

This is what we use at our lab

1.	Immerse slides in 95% alcohol in coplin jar for a min of 10 seconds.
2.	Rinse gently in running tap water for 10 seconds. 
3.	Place slides in Richard Allen Hematoxylin 7211 for 40 seconds.
4.	Rinse slides in tap water -5 dips.
5.	Rinse slides in tap water -5 dips.
6.	Wash slides in Bluing Reagent -Shandon Cat.#  9990176 10 dips.
7.	Place slides in tap water -5 dips.
8.	Place slides in 1% Alcoholic Eosin stain -10 dips.
9=2E	Place slides in 95% alcohol , 2 changes, -5 dips each.
10.	Place slides in 100% alcohol, 2 changes, -10 dips each.
11.	Place slides in Clear Rite (xylene substitute), 2 changes, -10 dips each.
12.	Coverslip slides with permanent mounting media, compatible with Xylene.

=0A-Hope it helps
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supv.
Laboratory, Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
(215) 938-3689

LeVier, Rebecca J wrote:
> Hi Folks, 
> I need some help, please.
=0A> Would someone be willing to share reagents and times of the H&E stain
=0A> for frozen sections if you are using a xylene substitute?
> We are having a hard time clearing....
> Thanks so much in advance.
> Becky
> Rebecca J. LeVier BS HT(ASCP)
> Histology Supervisor
> Email:
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Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
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