RE: [Histonet] A question on bone sections

From:"Monfils, Paul"

If by a "regular microtome blade" you mean a standard thickness disposable blade, then you will probably have difficulties. Standard disposable blades are too thin to cut very dense tissues without flexing. I do a lot of bone work here , on mice and larger animals. Using a standard disposable blade on bone will often result in chatter or "venetian blind" effect, and sometimes chipping out pieces from the block.  For bone I use Surgipath thick blades.  They twice as thick as standard blades and much more rigid.

> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of Yu, Jian
> Sent: 	Thursday, January 25, 2007 7:06 AM
> To: 	histonet@lists.utsouthwestern.edu
> Subject: 	[Histonet] A question on bone sections
> 
> Dear all,
> 
> We have parrafin embedded bone of mouse tibia (decalcified).  Can
> sections (longitudinal) of the bone be cut with a regular microtome
> blade or a special blade? We intend to do H&E staining on these sections
> and cut soft tissue sections (mouse) in our own lab routinely.  
> 
> This is the first time we are dealing with bone.  Any advice will be
> very helpful and appreciated.
> 
> Thanks, Jian
> *******************************************************************
> Jian Yu, Ph.D.
> University of Pittsburgh Cancer Institute
> Phone: 412-623-7786
> Email: yuj2@upmc.edu
> *******************************************************************
> 
> 
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