RE: [Histonet] A question on bone sections

From:"DiCarlo, Margaret"


Yes, you can cut the decaled mice tibia with a disposable or regular
microtome blade.  Before cutting, I soak a few of the blocks in decal,
in an empty slide box.  Set it on top of a block of ice and add some
water, soak for about 10 minutes, rinse block under cold water and then
it is ready to be cut.

Peggy DiCarlo HT (ASCP)
Orthopedics Bone Lab
Buffalo General Hospital 
Buffalo, NY  14203

-----Original Message-----
[] On Behalf Of Yu, Jian
Sent: Thursday, January 25, 2007 10:06
Subject: [Histonet] A question on bone sections

Dear all,

We have parrafin embedded bone of mouse tibia (decalcified).  Can
sections (longitudinal) of the bone be cut with a regular microtome
blade or a special blade? We intend to do H&E staining on these sections
and cut soft tissue sections (mouse) in our own lab routinely.  

This is the first time we are dealing with bone.  Any advice will be
very helpful and appreciated.

Thanks, Jian
Jian Yu, Ph.D.
University of Pittsburgh Cancer Institute
Phone: 412-623-7786

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