[Histonet] testicles

From:Inga Hansson

Hi everyone,

A collegue of mine is trying to section frozen rat testicle (perfused 
and cryoprotected in sucrose) at -30°C. Is that a good temperature to 
use? The sections seem fragile and tend to break. They´re 10 microns 
thick. Or is it bad fixation?

Thanks in advance for any help!


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