[Histonet] paraffin sectioning troubleshooting

From:William Ares

As a newbie to paraffin sectioning on a paraffin microtome, I've been plagued with differential results since starting.  My current issue is that as they're cut, my sections are rolling back up on themselves instead of coming off the blade with the appearance of a venetian blind (as they were when the sections were looking good).  As they appear brittle, I've tried putting the blocks in a humid environment to try to rehydrate them a little, but to no avail.  Anyone have any good troubleshooting tips for me?  Thanks in advance.

William Ares
Research Technician
Laboratory of Molecular and Developmental Neuroscience
Weill Medical College of Cornell University
Tel: (212) 746 5056
Email: wia2005@med.cornell.edu

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