Histonetters,

 

A while ago I remember someone mentioning a method to soften bone after
it's been decaled but prior to processing.  Can anyone tell me the
procedure and does it have any affect on the H&E staining?  Lately, my 5
x 7" size bones have been giving me great difficulty when sectioning
since they have been very dense and hard.   I process with xylene.  

 

Thanks for your help.

 

Peggy DiCarlo HT (ASCP)

Orthopedics Bone Lab

Buffalo General Hospital

100 High St.

Buffalo, NY  14203

716-859-1293

  



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