[Histonet] Trying to get consensus - Frozen section immediate post fixation or air dry

From:"John MacDougall"

Hi Histonet

I have seen two basic camps regarding the treatment of frozen sections
once the section hits the slide - One camp fixes immediately and one
camp leaves the section to air dry, sometimes overnight.  I realize that
there is likely not a "right" answer, but are there advantages to one
process method over another? I'm in the midst of trying out both
processes and evaluating myself, but in the meantime I'd like to hear
feedback from the community.






John MacDougall, Ph.D.

Lead Senior Scientist

Infinity Pharmaceuticals

780 Memorial Drive

Cambridge, MA



Voice - 617.453.1214

Fax - 617.453.1001


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