[Histonet] Re: procedure to xray fixed breast tissue
We use large culture dishes from Micro and put the smaller of the two on top of the larger to hold the tissue flat. I went to the Dollar Store and bought tortilla warmers and put Biohazard stickers on them to use for transporting the culture dishes back and forth from Radiology.
The fluids are kept intact and the specimen is covered during transport.
>>> 01/25/07 12:01 PM >>>
Cita Ball asks:
I would be grateful for any information to help us set up a procedure to
x-ray [fixed] breast tissue here at Scarborough hospital [in the UK]. Any ideas on
what is the best type of board to fix the slices of breast to and to cover
them with to keep them in the same position to enable the orientation to be
maintained whilst being x-rayed, would be greatly appreciated.
A surgical pathologists needs to be prepared to X-ray unfixed and fixed wet
tissue, as well as paraffin blocks. Usually it's best to lay the tissue down on
a piece of plastic (such as a plastic tray) and take the tray to the
radiology department and get a specimen radiogram done on it. It's helpful to phone
ahead, and if you haven't done it before, you should talk to a radiologist about
It's a good thing to fix the tissue as flat as possible - on a piece of
cardboard - if you anticipate getting specimen radiography done after fixation.
A lot of such procedures can be avoided if the radiographers can be persuaded
to send the original specimen radiogram along with the wire localization
specimen. For convenience they can shoot two films (since there is no radiation
exposure to the patient) so that the pathology service can discard the film
rather than take the trouble to return and re-file it.
You need to have a view box - holding the film up to a fluorescent light and
squinting isn't satisfactory. If you can't have a view box (the red-haired=20
stepchild again) go to a photography supply store and buy one of the light units
photographers use for looking at negatives and cutting film.
Scarborough - is that where they cook chicken with parsley, sage, rosemary,
and thyme... the renowned Scarborough Fowl... don't throw that view box at
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