[Histonet] Paraffin sectioning troubleshooting
How thick are your sections? Have you tried adjusting your knife angle? I
keep my blocks in the refrigerator the day I am sectioning, and take them out
just before sectioning. I allow them to warm for a few minutes, and ribbon
off my sections. If I start having problems, I put a frozen artificial ice
pack on the block surface for 30-60 seconds, allow to warm for a few seconds,
then section again.
The brittleness of your sections sounds like a processing issue. You may
need to adjust your processing schedule if the tissues are brittle, especially
if you are using an automatic processor. If the wax is brittle, try another
wax formulation with additional plasticizers that serve to "soften" the wax.
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