[Histonet] MGH Neuropath
I'd like to let everyone know that MGH Neuropath moved, but never liquidated!
Here are some thoughts on Hydroquinone & Lithium Carbonate: Tim, the MGH
protocol says that either Hydroquinone or Lithium Carbonate can be used. I
think that came from the original lab handbook by DR's Kubik & Richardson. The
Kluver-Barrera method uses Lithium Carbonate as from the 1957 AFIP manual and
the Holmes Luxol Fast Blue uses the Hydroquinone & Sodium Sulfite. This was a
step taken from Bodian procedure.
I started using Lithium Carbonate several years ago when I went to use the
Hydroquinone/sodium sulfite solution, I discovered that it had turned brown. I
didn't have time to remake the solution, so I used the Lithium Carbonate and it
worked beautifully. Maybe Kubik & Richardson had the same emergency,
I use a super saturated Lithium Carbonate stock solution. Dilute 1:1 before
use and filter and use for differentiating the slides after their incubation in
Luxol Fast Blue. Harris hematoxylin for 3 min, acid ETOH, lithium carbonate
again and then 1% alcoholic eosin with 0.5% acetic acid for 3 min and then into
100% ETOH x 3, xylene x 3.
Gill hematoxylin gives poor results for this stain.
One last note: pay attention to the washing steps after acid alcohol & lithium
carbonate. If not washed for a few minutes between steps, the slides will fade
Holly A. Goolsby, HT(ASCP)
Sr. Tech Specialist, Neuropathology
Pathology, Blake 350 B
Massachusetts General Hospital
Boston, MA 02114
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