[Histonet] Double staining with Mouse Mabs on Ventana discovery
Hi Everyone,
I'm wondering if anyone has experience double-staining using 2 mouse
monoclonal antibodies (IHC, one for CD4, one for FoxP3) on human FFPE
tissue using the Ventana Discovery system? Do you have any protocols
you could share? Also, I already have the DAB-MAP kit in house and
would like to use that in the detection rather than buying 2 new
detection kits, which Ventana detection kit should I use for best
contrast and practicality (slides mounted using Cytoseal 60 following
alcohol and xylene).
Any suggestions would be much appreciated!
Katy Milne
Deeley Research Centre
BC Cancer Agency
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>