[Histonet] Blue Nevus Diease-Help?

From:Scheherazade Humphrey

 
  Sorry I forgot to change the subject line for my message. Can anyone help me find a IHC stain the is performed on the disease Blue Nevus. I am having a hard time finding out if Fontana Masson is the correct one to use and also a possible case study to reference this 
disease to. I had to reasearch this disease for a school project. If anyone could help me, your participation would be greatly appreciated. Thank You. 


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Today's Topics:

1. RE:Information on Antibodies (Mark Elliott)
2. CK 19 (Dana Settembre)
3. Re: Help with a Reichert Jung Frigocut 2800
(Don.Birgerson@leica-microsystems.com)
4. RE: Microwave procedure for monitoring temperature rep
roducibility (hymclab)
5. Re: Microwave procedure for monitoring temperature
reproducibility (Phil McArdle)
6. RE: Microwave procedure for monitoring temperature rep
roducibility (Inman, Anna)
7. RE: Microwave procedure for monitoringtemperature
reproducibility (Morken, Tim)
8. Re: Histonet Digest, Vol 38, Issue 26 (Scheherazade Humphrey)
9. RE: Re: Histonet Digest, Vol 38, Issue 26 (Terri Braud)
10. RE: Microwave procedure for monitoringtemperature
reproducibility (Chaussey, Leslie)


----------------------------------------------------------------------

Message: 1
Date: Thu, 18 Jan 2007 06:21:32 -0800
From: "Mark Elliott" 
Subject: [Histonet] RE:Information on Antibodies
To: ,
Message-ID: <45AF11EC020000D6000306CE@mail.mrl.ubc.ca>
Content-Type: text/plain; charset=US-ASCII

Marsha
I have used D2-40. What sort of info are you looking for?
MArk

>>> "Mildred Fail" 1/17/2007 10:01:10 AM >>>
Marsha
We use

2. Mesothelial clone HBME-1 from DAKO IVD AB 
3. CK 19 clone b170 fromVector RUO Ab

Rena Fail

>>> "mprice26@juno.com" 01/17/07 12:15PM >>>
Hello in Histoland,
I need information on the following antibodies:

1. D2-40

2. HBME-1

3. CK-19

Thank you in advance.

Marsha Price, HT, QIHC (ASCP)


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------------------------------

Message: 2
Date: Thu, 18 Jan 2007 09:36:28 -0500
From: "Dana Settembre" 
Subject: [Histonet] CK 19
To: , , "Mark
Elliott" 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

Hello Marsha,
I use CK 19 on FFPE human tissue.
I use Dako's cat.# M0888 
It is an In Vitro Diagnostic antibody.
I use it at a dilution of 1:25 and incubate 15 mnutes RT
I also use their Target Retreival Solution
and use a papillary carcinoma as a positive control.
I get nice results.
Good Luck


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ USA


>>> Mark Elliott 01/18/07 9:21 AM >>>
Marsha
I have used D2-40. What sort of info are you looking for?
MArk

>>> "Mildred Fail" 1/17/2007 10:01:10 AM >>>
Marsha
We use

2. Mesothelial clone HBME-1 from DAKO IVD AB 
3. CK 19 clone b170 fromVector RUO Ab

Rena Fail

>>> "mprice26@juno.com" 01/17/07 12:15PM >>>
Hello in Histoland,
I need information on the following antibodies:

1. D2-40

2. HBME-1

3. CK-19

Thank you in advance.

Marsha Price, HT, QIHC (ASCP)


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------------------------------

Message: 3
Date: Thu, 18 Jan 2007 08:44:44 -0600
From: Don.Birgerson@leica-microsystems.com
Subject: Re: [Histonet] Help with a Reichert Jung Frigocut 2800
To: Kurt Musselmann 
Cc: histonet@lists.utsouthwestern.edu,
histonet-bounces@lists.utsouthwestern.edu
Message-ID:


Content-Type: text/plain; charset=US-ASCII

Hello Kurt,
I believe we still have anti roll plates for most of our cryostats. I
will call you on the phone so we can play a game of twenty questions to
identify your holder.
Best regards,
Don

Don Birgerson
Leica Microsystems
Technical Assistance Center
Don.Birgerson@Leica-Microsystems.Com
1-800-248-0123 ext 5918 (7:00 to 4:00 CT)



Kurt Musselmann 
.nih.gov> To 
Sent by: 
histonet-bounces@ cc 
lists.utsouthwest 
ern.edu Subject 
[Histonet] Help with a Reichart 
Jung Frigocut 2800 
01/18/2007 08:06 
AM 







Hello everybody
I am new to the list, and was directed to you guys by Alan Bright.

I have started using a Reichart Jung Frigocut 2800, and we have a badly
chipped anti-roll plate. Our plate measures roughly 5x3x0.3 cm, and I am
trying to find a replacement for it.

Does anybody know of a company that manufactures anti-roll plates of that
size, or would I have to change the whole anti-roll device (I had ordered
one from Electron Microscope Sciences (EMS) but it did not fit our machine
and had to be returned.

I appreciate any input


Kurt Musselmann
--
Kurt Musselmann Ph.D.
NIDCR/NIH
30 Convent Drive
Building 30, Room 403
Bethesda, MD 20892-4370
(301) 496 3065



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------------------------------

Message: 4
Date: Thu, 18 Jan 2007 08:51:49 -0600
From: hymclab 
Subject: RE: [Histonet] Microwave procedure for monitoring temperature
rep roducibility
To: 'Zummak Melissa' ,
histonet@lists.utsouthwestern.edu
Message-ID:


Content-Type: text/plain

There is a CLSI (formerly NCCLS) Standard and Guideline book available:
Microwave Device Use in the Histology Laboratory; Approved Guidelines.
(description: This document provides recommendations for reproducing the
performance of microwave-accelereated procedures to prepare biological
specimens in the histology laboratory).
Go to the CLSI website for info: www.clsi.org.

I found this very helpful in fulfilling the new CAP requirements.

Dawn D. Schneider, HT(ASCP)
Howard Young Medical Center
Woodruff, WI 54568
(715)356-8174
schneiderd@hyhc.com


-----Original Message-----
From: Zummak Melissa [mailto:Melissa.Zummak@ssfhs.org] 
Sent: Wednesday, January 17, 2007 10:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microwave procedure for monitoring temperature
reproducibility

Hi everyone,

Does anyone know where I can find recommendations for checking\monitoring
temperature reproducibility for microwaves, I only use the microwave for
certain special stains currently.

Melissa Zummak
Alverno Clinincal Lab
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------------------------------

Message: 5
Date: Thu, 18 Jan 2007 10:24:00 -0500
From: Phil McArdle 

Subject: Re: [Histonet] Microwave procedure for monitoring temperature
reproducibility
To: Zummak Melissa 
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <45AF9110.8080104@ebsciences.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hello everyone:

And now a vendor weighs in:

We have several calibration and "accreditation-related" articles 
available on our website (www.ebsciences.com , 
http://www.ebsciences.com/papers/microwave_quality.htm , 
http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf ).

In addition, we're happy to see that microwave use in the histology 
laboratory is finally being given the scrutiny it deserves. Many of the 
suggestions in CLSI's GP-28 guidelines (referenced in other postings) 
are excellent, for example, troubleshooting, container placement, power 
output determination, and overall, standardization of procedures and 
elimination of variables. However, I feel there are some significant 
problems with this publication; I have previously informed CLSI of my 
primary concern:

Page 11, section 4.7: "A qualified authority CERTIFIED IN USING 
MICROWAVE LEAKAGE INSTRUMENTATION [my emphasis] should determine 
microwave leakage..." The problem is, to our knowledge, NO SUCH 
CERTIFICATION EXISTS! Furthermore, despite repeated requests, CLSI has 
not been forthcoming with suggestions. This is not to imply the absence 
of extant legislation, Federal and otherwise, regulating the manufacture 
and use of microwave devices; manufacturers have to comply with FDA and 
other regulations regarding microwaves intended for in-vitro diagnostic 
use. But as for leakage detection in the field, we have yet to come 
across any relevant (or irrelevant, for that matter) Federal or State 
certification. As you can imagine, for institutions deciding to adopt 
the more stringent CLSI guidelines, this poses something of a "Catch-22."

In addition, page 17 outlines the use of a neon bulb array, for those 
who aspire to eliminate "hot spots" in the microwave chamber. However, 
while this can be a "fun" way to indirectly observe microwave energy, 
unless the microwave were horribly defective, it is a fairly useless 
exercise. Any object (including the array itself) placed in the 
microwave chamber affects the distribution of microwaves, a classic 
example of attempted observation affecting the phenomenon observed.

I want to stress, however, that overall, publication GP-28A is an 
outstanding effort to provide "recommendations for quality assurance and 
safety procedures for microwave equipment use" in their words, and to 
help troubleshoot and eliminate variability in results of microwave usage.

Best regards,

Phil McArdle
--

Microwave Product Manager

Energy Beam Sciences, Inc.
29-B Kripes Rd.
East Granby, CT 06026

Tel: 800.992.9037 x 341
Fax: 860.653.0422

pmcardle@ebsciences.com
www.ebsciences.com

You must be the change you want to see in the world.
- Mahatma Gandhi

NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it; please do the right thing and make it go away. Thank you.


Zummak Melissa wrote:
> Hi everyone,
>
> Does anyone know where I can find recommendations for
> checking\monitoring temperature reproducibility for microwaves, I only
> use the microwave for certain special stains currently.
>
> Melissa Zummak
> Alverno Clinincal Lab
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


-- 





------------------------------

Message: 6
Date: Thu, 18 Jan 2007 08:50:47 -0700
From: "Inman, Anna" 
Subject: RE: [Histonet] Microwave procedure for monitoring temperature
rep roducibility
To: "hymclab" , "Zummak Melissa"
, 
Message-ID:
<2925AE271EAAD440AF48FCCEB8002D090372F4C3@smgmail01.smgj.sclhs.net>
Content-Type: text/plain; charset="us-ascii"

What I recall about that publication......it doesn't really address
microwave usage for anything other than a specimen processing angle;
whereas, labs like mine only use it for heating agar/histogel to make
cell blocks and things........



Anna Inman, HT(ASCP)

Clinical Resource Specialist

SMH Pathology



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab
Sent: Thursday, January 18, 2007 7:52 AM
To: 'Zummak Melissa'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave procedure for monitoring temperature
rep roducibility



There is a CLSI (formerly NCCLS) Standard and Guideline book available:

Microwave Device Use in the Histology Laboratory; Approved Guidelines.

(description: This document provides recommendations for reproducing the

performance of microwave-accelereated procedures to prepare biological

specimens in the histology laboratory).

Go to the CLSI website for info: www.clsi.org.



I found this very helpful in fulfilling the new CAP requirements.



Dawn D. Schneider, HT(ASCP)

Howard Young Medical Center

Woodruff, WI 54568

(715)356-8174

schneiderd@hyhc.com





-----Original Message-----

From: Zummak Melissa [mailto:Melissa.Zummak@ssfhs.org] 

Sent: Wednesday, January 17, 2007 10:34 AM

To: histonet@lists.utsouthwestern.edu

Subject: [Histonet] Microwave procedure for monitoring temperature

reproducibility



Hi everyone,



Does anyone know where I can find recommendations for
checking\monitoring

temperature reproducibility for microwaves, I only use the microwave for

certain special stains currently.



Melissa Zummak

Alverno Clinincal Lab

_______________________________________________

Histonet mailing list

Histonet@lists.utsouthwestern.edu

http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE: This e-mail communication and any attachments
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------------------------------

Message: 7
Date: Thu, 18 Jan 2007 11:33:53 -0500
From: "Morken, Tim" 
Subject: RE: [Histonet] Microwave procedure for monitoringtemperature
reproducibility
To: 
Message-ID:


Content-Type: text/plain; charset="us-ascii"

Phil wrote: "Page 11, section 4.7: "A qualified authority CERTIFIED IN
USING MICROWAVE LEAKAGE INSTRUMENTATION [my emphasis] should determine
microwave leakage..." The problem is, to our knowledge, NO SUCH
CERTIFICATION EXISTS! Furthermore, despite repeated requests, CLSI has
not been forthcoming with suggestions."

That's funny, but brings up an interesting question: Who does oversight
on a group like CLSI? If they went to the trouble of researching this
guideline, how is it that they never (apparently) realized there is no
such certification. What made them write such a requirement without
knowing if such a certification exisits? Did they just assume it
existed? And, if they did any lab work with an actual microwave, how
could they have done so without first having it checked by a person
certified to do so? 

The final question. If CLSI can't figure that out, should we believe
them on anything else? This is a private group that has set itself up
as the final word on laboratory standardization. They charge massive
amounts of money for rather average informational documents (most of
which, these days, anyone can find on the internet for free with a
little persistance) and then something like this makes them look like
fools. It seems the only possible oversight is to refuse to buy
defective documents.

Actually, it kinda reminds me of another body that dreams up fantasy
questions to torture lab managers with...

Tim Morken



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phil
McArdle
Sent: Thursday, January 18, 2007 7:24 AM
To: Zummak Melissa
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Microwave procedure for monitoringtemperature
reproducibility

Hello everyone:

And now a vendor weighs in:

We have several calibration and "accreditation-related" articles
available on our website (www.ebsciences.com ,
http://www.ebsciences.com/papers/microwave_quality.htm ,
http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf ).

In addition, we're happy to see that microwave use in the histology
laboratory is finally being given the scrutiny it deserves. Many of the
suggestions in CLSI's GP-28 guidelines (referenced in other postings)
are excellent, for example, troubleshooting, container placement, power
output determination, and overall, standardization of procedures and
elimination of variables. However, I feel there are some significant

=== message truncated ===


Scheherazade H. 
 
---------------------------------
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