[Histonet] A question on bone sections

From:"Yu, Jian"

Dear all,

We have parrafin embedded bone of mouse tibia (decalcified).  Can
sections (longitudinal) of the bone be cut with a regular microtome
blade or a special blade? We intend to do H&E staining on these sections
and cut soft tissue sections (mouse) in our own lab routinely.  

This is the first time we are dealing with bone.  Any advice will be
very helpful and appreciated.

Thanks, Jian
Jian Yu, Ph.D.
University of Pittsburgh Cancer Institute
Phone: 412-623-7786
Email: yuj2@upmc.edu

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