Re: [Histonet] (no subject)

From:Rene J Buesa

Gloria:
  The only thing is that you will have to end doing the procedure manually and your only concern would be to make sure to use enough reagents to cover the whole section.
  At the end you just will have a more expensive procedure, but other than that you could complete it using any standard protocol, from the time and dilutions points of view.
  René J.

"gloria @home"  wrote:
  Hi gurus,

I would like to perform whole-mount immunohistochemistry on mouse/rat 
embryos. However, they are bigger than usual. ~E15.

Does anyone have a detailed protocol of the typical tools to use solutions, 
etc., or any particular modifications to be made to already existing 
protocols?

Bearing in mind that they might be too big to mount onto a normal slide, or 
even a standard depression slide, and there might be a problem with 
visualization with confocal.

Thanks,

powerhungrymice

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