Re: [Histonet] Tumour endothelium - Help!

From:Rene J Buesa

My experience is with Ulex europaeus lectin. Would you consider using it instead of the "tomato" lectin?
  If so I can send you my protocol.
  René J.

"Martin S."  wrote:
Hi all,

I'm working on some human tumours which have been grown in mice and I
want to stain the endothelium. Someone gave me an aliquot of tomato
lectin which they use for brain tissue. I tried it on two types of
tumour without much success (both were FFPE);

human melanoma in mouse lung - stained the alveoli and inside layer of
the bronchioles, little staining of the melanoma

human B cell lympoma - staining throughout the tumour but no staining of
what looks like blood vessels

Does anyone have any experience using tomato lectin? Does this sound
like background staining of does the tomato lectin bind to other cell

What does anyone else use for endothelium staining of tumours?


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