Depends on what you are staining for - are you looking for a good 
general stain? There are several protocols in the Ruzin book, Plant 
Microtechnique and Microscopy.

Andi Grantham

-----Original Message-----
From: EWURDAK@CSBSJU.EDU
To: histonet@lists.utsouthwestern.edu
Sent: Wed, 10 Jan 2007 3:33 PM
Subject: [Histonet] Staining plant tissues

Hello Histonetters,
I am looking for a good stain for plant tissue.  A couple of years ago 
we
tried Johansen's quadruple stain as described in Gray's Handbook of 
Basic
Microtechnique, but we were not satisfied with the results.  We fixed 
the
tissues in FAA (95% ethanol, glacial acetic acid, formalin).
Thank you in advance for any advice you can give me.
Liz

Elizabeth Wurdak, PhD
Biology Department
Saint John¹s University
Collegeville, MN 56321
Tel: (320) 363-3177


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