Re: [Histonet] Perfusion fixation of pig lungs

From:Geoff McAuliffe

Hi Lene:

1. Decide if you want to perfuse via the vascular system (washing out 
the blood) or instill fixative via the trachea and bronchial tree.  Note 
that the position of the animal during instillation will determine which 
regions of the lung are fixed first (or at all!). Study of the anatomy 
of the bronchial tree of the pig will pay dividends.
2. Decide if you are going to look at the entire lung or just one (or 
more) small regions. I suggest looking at one bronchopulmonary segment 
from one (or more?) lobes to conserve resources and minimize variation 
from animal to animal. If that is your decision then you can do a little 
dissection and either perfuse that segment or instill into that segment.
    Good luck!


LNLJ (Lene Lyngsie Jensen) wrote:

>I hope that someone here can help me.
>We are planning to do some research in pig lungs. Due to this
>investigation it is very important to have a fast and effective
>We will therefore try to make a perfusion fixation. But a pig is a very
>large animal and therefore we would like to limit the fixation only to
>the lung region, and not a full body perfusion fixation.
>Is there anyone how has experience with perfusion fixation limit to only
>one organ in pigs? 
>All suggestion will be read with enthusiasm.
>Experience from similar fixation methods in other animals is welcome as
>Lene Lyngsie Jensen
>Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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