Re: [Histonet] HOPE fixation

From:"Bryan Hewlett"


HOPE =Hepes-glutamic acid buffer mediated Organic solvent Protection Effect.
Tissues are placed in this non-fixative buffer at 4C O/N, then 
fixed/dehydrated  in acetone and transferred to paraffin.

Here is a website


----- Original Message ----- 
From: "Kim Merriam" 
To: "Histonet" 
Sent: Friday, January 05, 2007 2:13 PM
Subject: [Histonet] HOPE fixation

OK, I know someone on this list must know the answer to this.  What is HOPE 
fixation?  I am reading a journal article that refers to this procedure.  I 
have done a Pubmed search, and I can find several references to it, but no 
actual protocol on how to make the stuff up (maybe you need to buy it?).

I am not sure if I would ever be trying this method, but inquiring minds 
want to know!


Kim Merriam, MA, HT(ASCP)
Cambridge, MA

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