Re: [Histonet] CD133 Help!!

From:Rene J Buesa

  I have not worked with this Ab but as a rule, when I did not get a clear reaction, I used to work with the HIER pH or the dilution, rather than with the incubation time. No matter how long you increase the reaction time, if there is not enough Ab concentration (or epitope signal for that matter) you will not get an intense result.
  René J.

"Tarango, Mark"  wrote:
  If anyone has a protocol for CD133 on paraffin OR frozen tissue that
works, please send it to me! I've tried so many times w/ this antibody
and I'm having a hell of a time getting any staining.


Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV 89135 

Direct Line (702) 822-5112

Fax (702) 939-7663

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