Rat anti-mouse B220 from BD Pharmingen works like a charm in frozens 
and PARAFFIN. I promise :)

Good luck,
Andrea


At 2:40 PM -0800 1/11/07, Milne, Katy wrote:
>  Hi everyone, not sure if this worked last time so I'll try again...
>
>I have to look for an antibody to detect B cells in mouse formalin-fixed
>paraffin embedded tissue and I'm not looking forward to it given the
>troubles I've had with some other CD markers.
>
>Is there anyone that could recommend a reliable antibody, preferrably
>not a mouse monoclonal as I'd like to avoid the extra cost of bringing
>in a MOM kit.  I was thinking of using CD19 but if anyone can suggest
>another reliable marker that isn't only present on subsets of cells it
>would be much appreciated.
>
>Thanks,
>Katy Milne
>Deeley Research Centre

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