RE: [Histonet] ISH protocol with DIG-labeled probes

From:"Liz Chlipala"


Try Genedetect website it has a lot of good info
on ISH protocols.  We're working on some biotinylated probes from them right
now, but we have only tried one run and we got no staining so far.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of Andrea
Sent: Wednesday, January 03, 2007 4:52 PM
To: Histonet
Subject: [Histonet] ISH protocol with DIG-labeled probes

Dear All,

I was wondering if someone has a tried and true ISH protocol (I know, 
a stretch!) which they could send to me for paraffin sections using 
DIG labeled probes? I have tried some homemade mix-and-match 
protocols and am at my wits end getting the specificity to be 
accurate. I know my probes are good as I use them for other purposes 
with good success so that isn't it.


Thank you in advance,

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