RE: [Histonet] CD133 Help!!

From:"Patsy Ruegg"

Which cd133 are you using?  If you are using the antibody from Miltyeni I
suggest you toss it and get the one from Abcam rab polyclonal ab19898, I use
this at 1:100 overnight after HIER steam in HpH buffer for 25 min. with rab
labeled polymer hrp detection for 45 min. and dab for 15 min.

This antibody as well as many others has been discussed on the NSH IHC
Resource Group list serve, to join this group go to and submit
a membership form online.  We have a list serve similar to histonet that
focuses just on IHC/Molecular Diagnostics isssues.

Patsy Ruegg
Chair, IHCRG

-----Original Message-----
[] On Behalf Of Makhijani,
Nalini S
Sent: Wednesday, January 17, 2007 9:08 AM
To: Tarango, Mark;
Subject: RE: [Histonet] CD133 Help!!

Try letting it go overnight.
Nalini Makhijani

-----Original Message-----
[] On Behalf Of Tarango,
Sent: Tuesday, January 16, 2007 5:54 PM
Subject: [Histonet] CD133 Help!!

If anyone has a protocol for CD133 on paraffin OR frozen tissue that
works, please send it to me! I've tried so many times w/ this antibody
and I'm having a hell of a time getting any staining.






Mark Adam Tarango HT(ASCP)

Histology/Immunohistochemistry Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV 89135  

Direct Line (702) 822-5112

Fax (702) 939-7663



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