RE: [Histonet] Bacteria on the slides

From:"Liz Chlipala"

Could be in your water bath, do you change and clean your waterbaths daily?


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of eileen dusek
Sent: Tuesday, January 16, 2007 1:21 PM
To: histonet
Subject: [Histonet] Bacteria on the slides

Hi Everyone,
  We are having a major problem with AFB, Giemsa and know Fe staining. On
the slides its self, outside the tissue, we are getting AFB, Giemsa and Fe
staining. The AFB is brought down to water by hand, AFB and Giemsa are
stained on a staining rack. Our stainer recently had the water filter
changed. This is occurring with all the Techs. We use charged slides for all
  Could this be in the paraffin? The charged slides?
  All suggestions are appreciated.
  Edward Hospital

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