[Histonet] big tissue whole mount immunohistochem

From:"gloria @home"



>
>Hi gurus,
>
>I would like to perform whole-mount immunohistochemistry on mouse/rat 
>embryos. However, they are bigger than usual. ~E15.
>
>Does anyone have a detailed protocol of the typical tools to use solutions, 
>etc., or any particular modifications to be made to already existing 
>protocols?
>
>Bearing in mind that they might be too big to mount onto a normal slide, or 
>even a standard depression slide, and there might be a problem with 
>visualization with confocal.
>
>Thanks,
>
>powerhungrymice
>
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