Dear Histonetters,

I have been working with Rabbit polyclonal to YAP1 from ABCAM.  I have
tried two breast cancer specimens and one live tumor and have not seen
any staining of the paraffin sections.  I used antigen retrieval and a
1:20 dilution of the primary antibody.  Is there any special tricks to
make this antibody work?  Has anyone done this on an automated stainer?

Thanks for any help in advance
Elaine Dooley
HTL 
Shands Teaching Hospital
352-265-0111 ext 72117

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