Thanks to everyone for your replies; just one more problem - its mouse
endothelium I want to look at rather than human.
 
I think the problem with the tomato lectin is cross-readtivity with
epithelial cells.
 
I have found an anti-mouse Factor VIII antibody from Abcam but its quite
expensive.....budgets!!
 
Most of the tissues are FFPE and I cant find an anti-mouse CD31 antibdy
reported to work realiably in paraffins.
 
Any more suggestions?
 
Cheers
Sonya
 


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