Andrea,

Please tell me what sort of problems you are having, and are you using
DIG labeled oligo or riboprobes?

We have the Ventana Discovery instrument for our slide-mounted ISH
samples. However, we've adapted a benchtop protocol to work with their
unit. We primarily use riboprobes and if this is what you are using,
I'll be happy to share our protocol with you.

Best wishes,

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110

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