[Histonet] Re: Bone Marrow stain
Jennifer Cresor (where?) asks about bone marrow staining.
If you're staining smears, all the Romanovsky (polychrome) type stains work
the same way - Giemsa, Wright, what have you. Smear preparation, time staining,
and other details of technique are very important. It's particularly
important to avoid even the slightest water contamination of your stock bottle of
stain. I expect your pathologists to know these things and to carefully supervise
your technique until the results are satisfactory (dream on!)
If you're staining tissue sections - I don't know of any Romanovsky stain
method that works on formalin fixed tissues. Back in the days when dinosaurs
walked the earth, we fixed the tissue in Zenker's or Helly's fixative (contains
both mercury and chromium. B-5 doesn't work) and did the old Wolbach Giemsa
stain - I can give you more information on that if you're interested.
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