[Histonet] Microinjection whole-mount immunolabelling
Hi gurus,
Another question, what's the deal with this microinjection whole-mount
immunolabelling stuff? I read in a textbook that by injecting the Ab
directly into your embryos, you increase penetration and it works even if
the Ab never labelled during conventional whole mount immunohistochemistry.
How time-consuming, tricky is it?
What are some important things I have to consider if i wish to try this out
just in case my conventional whole-mount ihc doesn't work?
I'd appreciate any help from people who've done this before!
thanks
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