[Histonet] Microinjection whole-mount immunolabelling

From:"gloria @home"

Hi gurus,

Another question, what's the deal with this microinjection whole-mount 
immunolabelling stuff? I read in a textbook that by injecting the Ab 
directly into your embryos, you increase penetration and it works even if 
the Ab never labelled during conventional whole mount immunohistochemistry.

How time-consuming, tricky is it?

What are some important things I have to consider if i wish to try this out 
just in case my conventional whole-mount ihc doesn't work?

I'd appreciate any help from people who've done this before!


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