[Histonet] Cryostats for insect neuroanatomy?
Hello,
I want to do silver staining in Drosophila and I have looked at a number of
published protocols. One thing I have noticed is that practically all of
them use
paraffin or plastics sectioning. The problem is that we have only a cryostat
in
the lab. So is there anything wrong with cryostats? For example, I have been
worrying that perhaps the 30% sucrose treatment will shrink and distort the
tissue? Thanks for your help!
Hao Li
Institute of Neuroscience, CAS
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