Re: [Histonet] Stains for Hemaglobin

From:Rene J Buesa

  There are several methods for hemoglobin:
  1- Ralph[Stain Technol., 16: 105 (1941)] for dry smears.Hemoglobinˇúdark brown.
  2- Goulliart [C.reend.Soc.Biol.,135: 1260 (1941)] for frozen sections or dry smears.
  3-Dunn [Arch.Pathol.,41: 676 (1946)] for frozen of paraffin sections of tissues
  fixed with NBF.Hemoglobin ˇú bue; nuclei ˇúred; cytoplasm ˇú pink.
  4- O'Brien [Stain Technol. 36: 57 (1961) for for whole mount embryos, hematopietic cells and electrophoresis.
  Hope this will help you.
  Ren¨¦ J.

"Randolph-Habecker, Julie"  wrote:
  I am looking for a specific stain for hemoglobin in formalin-fixed,
paraffin-embedded canine tissue. I could either use IHC or a special
stain. Any ideas?

I found a reference for a Puchtler-Rosenthal-Sweat Method for hemoglobin
but it says that it only works in tissue fixed in Zenker's.

Just wondering if anyone has any experience in this before we try it.



Julie Randolph-Habecker, Ph.D.
Staff Scientist - Manager
Experimental Histopathology Shared Resource
Fred Hutchinson Cancer Research Center
1100 Fairview Ave, N. M5-A803
Seattle WA 98109-1024

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