Re: [Histonet] RE: Eosin too pink

From:Jennifer MacDonald


   Eosin is differentiated in the dehydrating alcohols.  You ca= n extend
   the  time  in  the  alcohols to get a lighter pink.  Also contact= the
   tech  support  at  Richard-Allan.   There are two types of eosin that   they sell and one is really concentrated.

   Jennifer

   -----histonet-bounces@lists.utsouthwestern.edu wrote: ---= --

     To: = histonet@lists.utsouthwestern.edu
     From: Mark Adam Tarango 
     Sent  by: histonet-bounces@lists.utsouthwestern.eduDate: 01/14/2006
     06:03PM
     Subject: [Histonet] RE: Eosin too pink
     =  So  is  he  complaining that the eosin is too strong or just too
     pink?  I= t seems to me like the more water you have in the alcohol
     after  Eosin  or  if=  you  use  a  water rinse after eosin it gets
     pinker....it's  more  orange when = you have a higher percentage of
     alcohol  in  the  alcohol  after  eosin(just see= ms that way to me
     anyway).   If  it's just too strong, dilute it down wi= th alcohol.
     I  hardly  get  any specimens, so I don't like changing the = Eosin
     often,  i  just  keep  adding  alcohol  to  it when it gets low and
     crusted = around the edges..... still seems to work fine....
     Mark T.
     &g= t; Sent: Friday, January 13, 2006 10:42 AM
     > To: histonet@lists.utsou= thwestern.edu
     > Subject: [Histonet] Eosin too pink
     >
     > H= i List,
     >  We are currently doing our H & E stain by hand an= d using the
     >  Richard-Allan  7211  Hematoxylin  and  their  Eosin  Y.   = ;Our
     pathologist is
     >  still  complaining  of slides being TOO PINK. &nb= sp;I have cut
     the eosin step
     >  down  to  just  5  dips from 15 dips, and = still too pink.  The
     Eosin is very
     > rich.
     >
     > Thi= s is our protocol:
     >  Hydrate  to  water like normal (thru Clear Rite 3,= 100% and 95%
     Flex)
     > Hematoxylin- 2 mins
     > Rinsing in water u= ntil the water runs clear
     > Clarifier II- 30 dips
     > running wat= er -30 dips
     > Richard Allan Bluing - 30 dips
     > running water - = 30 dips
     > 95% Flex - 10 dips
     > Eosin-Y- 5 quick dips
     = > Thru 95%, 100% etc to xylene
     >
     >  Any  suggestions  on  a  pos= sible method change or a more mild
     Eosin.
     >  Complaint  seems  to  solely=  "TOO  PINK".   I  have  done some
     experimenting w/
     >  cutting  the eo= sin w/ 80% Flex- and it does calm it down a bit
     but, the
     >  Hemo looks= more purply then blue, almost periwinkle so not sure
     if that
     >  will  =  be  better or not- we still need to have him review the
     test slides.
     >= Any suggestions ?
     >
     > Helayne Parker
     > Histology Sectio= n Head
     > Skaggs Community Health Center
     > Branson, Missouri
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