Re: [Histonet] Lot to lot antibody validation

From:"Amy Porter"

We use a known postive control and run a system negative (no primary) with 
each new lot of primary that is received.  We only run it one time maybe at 
the dilution for the old lot then slightly higher and slightly lower 
dilution with the already known pretreatment.  Slides are then reviewed and 
the acceptable dilution for the new lot is recorded on an "Antibody 
Titration Log Sheet" signed off on by the pathologist or researcher to whom 
the work is being provided for.  Hope this helps out...
----- Original Message ----- 
From: "Connolly, Brett M" 
Sent: Friday, January 20, 2006 9:09 AM
Subject: [Histonet] Lot to lot antibody validation

>I would like to hear what procedures people are using to validate different
> lots of antibodies,  including # of specimens you might use for the
> validation.
> Thanks, Brett
> Brett M. Connolly, Ph.D.
> Merck & Co., Inc.
> MRL, Imaging Research
> WP-44K
> PO Box 4
> West Point, PA 19486
> PH 215-652-2501
> fax. 215-993-6803
> e-mail.
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