Re: [Histonet] Invertebrates in Sediment

From:Rene J Buesa

Bill: 
  "Invertebrate" is an extraordinarily encompassing zoological term and as such
  the techniques that can be used to "stain" its components are almost as
  numerous.
  Rose bengal has been used in many procedures one of which is to stain soil
  bacteria. Conn (1928) used the following recipe:
  Dist.water→100 mL + hydrated phenol→ 5 mL + calcium chloride → 10 mg +
  rose bengal → 1g.
  He used smears of gelatin suspended soil dried at 100║C stained on the slide and heated in a water bath x 1 min→ wash→dry.
  I don't think that your researcher wants to do this though. 
  It seems more likely that what the researcher wants is to stain and count 
  while suspended in liquid.
  If this is the case the technique will depend on the organisms been looked for.
  Perhaps you would be better off by using  iodine solution that will permit the
  visualization of many an invertebrate.
  Mix at a 50:50 rate Lugol's solution with a saturated aqueous solution of Eoin Y
  Mix 1 part of the solution with 10 parts of the sample.
  I hope this will help.
  RenÚ J.

BennettW@pac.dfo-mpo.gc.ca wrote:
  Hello Histonetters,

Thanks in advance for spending any time with this query! I have a
researcher that has collected sediment samples, preserved with NBF, and
wants to stain any invertebrates in the sediment so that they can be
enumerated and identified. The researcher wants to use rose bengal but
doesn't know at what concentration and neither do I. Can anyone help? 

Thanks
Bill Bennett
Histologist
Fisheries and Oceans Canada
Pacific Biological Station
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