Re: [Histonet] IHC losing tissue

From:Rene J Buesa

  You have a very good point there! I have been also told that the (+) slides
  after their expiration date can lose their properties. That fact did not come
  to my mind becasuse we were never confronted with it since we used all
  our (+) slides before the expiration date.
  This is something that should be considered also.
  René J. wrote:
About your IHC slides losing tissues during processing: It's probably what 
Rene and Linda said - preheating is necessary. But I have heard that the 
treated slides purchased from vendors lose their stickiness over time. You 
might check other lots or other vendors to see if you still have problems. 
Were these slides brand new? Has anybody else had problems with old 
pretreated slides losing their stickiness? 
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
Edmond, OK 73034
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