We use Dako's mouse monoclonal (clone OCH1E5) at a dilution of 1:200 for
30' (following HIER) with their EnVision+/DAB+ detection.  To be
perfectly honest, although normal hepatocytes stain well, I have been
disappointed with it's sensitivity in labeling hepatocellular
carcinomas.

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Martha Ward"  01/25/06 01:22PM >>>
I have approached by our Pathologists about working up the Hep Par 1
antibody and I was wondering if anyone out there is currently doing
it,
and if so, could you recommend a vendor, conditions, etc.  Thanks for
much in advance for any help you can give me.
 
Martha Ward, MT (ASCP) QIHC
Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
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