Re: [Histonet] Hematoxylin-eosin-saffron staining

From:Rene J Buesa

Hi Luminita:
  There is a procedure by Langeron (1942) for saffron and eosin B:
  Sol.A = 1% eosin; Sol.B = dist.water¡ú100mL + saffron¡ú2 g + + 5% aq. tanin¡ú1 mL + 40% formaldehyde¡ú 1 mL
  hydrated sections stained with hematoxylin ¡údifferentiated ¡ú nuclei blue ¡ú sol A for 5 min¡ú quick rinse in water ¡ú 70% ethanol for a few seconds ¡ú wash well in water ¡ú sol B for 5 min¡ú BLOT sections ¡ú flood the sections with absolute ethanol until dehydrated ¡ú xylene ¡ú cover slip.
  For the macrophages I would stain toluidine blue.
  Hope this will help.
  Ren¨¦ J.

"Castillos, Luminita"  wrote:

I am looking to find the protocol for the hematoxylin-eosin-saffron
method and I have difficulties. I would like to ask if anybody can share
with me this protocol?. I appreciated so much. Also, does somebody knows
a staining for macrophages from the lung or synovial tissue , using only
histochemistry staining without immunostaining?. Thank you again.


Luminita Castillos, Ph.D.
Research Scientist
Icoria, Inc., 
A Clinical Data Inc. company
108 T.W. Alexander Drive
P.O. Box 14528
RTP, NC 27709-4528
Phone: 919-425-2967
Cell: 919-302-0485

Effective Dec. 21, 2005, Icoria became a Clinical Data, Inc. company,
the worldwide leader in the molecular diagnostic field. The
Pharmacogenomics and the Molecular Services Division of Clinical Data,
Inc. (now with expanded genomics offerings from Icoria) specializes in
the development and commercialization of molecular biology and
pharmacogenomics solutions to biotechnology and pharmaceutical
companies, academia and government institutions.

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