Re: [Histonet] H.pylori immunos on gastric biopsies

From:Rene J Buesa

  I always used a phosphotungstic modification of the Steiner procedure to avoid
  the radioactive uranyl nitrate [J.Histotechnology, 24(2):113-6; 2001)
  I never considered doing IHC because of costs.
  The Steiner procedure cost was $0.91/slide and any "typical" IHC procedure
  would cost about $8/slide if done manually and about $6/slide if done automatic
  at low capacity.
  For me those were good reasons not to use IHC for H. pilorii.
  Hope this will help you.
  René J.

"Cazares, Ruth"  wrote:
  Hello Histonetters,

I was wondering if anyone out there is doing immuno staining routinely
on all gastric bx's? We are considering this in my lab, but I have my
reservations. If some of you do, how many a week? And those who don't,
why not? I will be doing a literature search on this topic, but if
anyone knows of a specific article I sure would appreciate it if you
could share it with me.

Thank you,

Ruth Cazares

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