Re: [Histonet] DIF Controls

From:Rene J Buesa

  We use to run positive controls for each antibody; this is the only way that you
  can be sure that your dilution worked.
  As positive controls we run positive cases with particularly high positive signal
  for each FITC conjugated antibody.
  If you don't do that you could end repporting a false negative since you don't 
  have the + control to compare with.
  Hope this helps!
  René J.

Steve Weaver  wrote:
  **New** CAP Question: ANP.21850 "Are appropriate positive and negative
controls performed with each case tested using immunofluorescence?"
We currently just run one (+) control, usually IgG or C3, for the entire
run that includes IgA, IgG, IgM, C3, C4 and fibrinogen. My question is
- should I be running (+) controls for all antibodies? Your thoughts
please. Please indicate if you have been inspected on this new
question. Thank you. sw

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