Patrick,
Preparing frozen sections of mouse eyeballs is difficult, especially if you 
would like to retain the morphology of the structures of interest.

We have been successful using the CryoJane Tape-Transfer system. Please 
visit the Instrumedics' web site for the details on the CryoJane method.

If you have any questions please contact us.

Bernice
Instrumedics
----- Original Message ----- 
From: "Patrick Laurie" 
To: 
Sent: Tuesday, January 17, 2006 12:36 PM
Subject: [Histonet] Cryosectioning mouse Eyeball


I have a couple of Researchers here who are interested in cryosectioning
mouse eyeballs to look at the corneas.  I have given them a few hints
(colder temperatures, cutting quickly and evenly, etc.) but they are
having variable results.  I was wondering if anyone has any further
hints.  Is there some sort of freezing media that someone would
recommend?  We are using Tissue-Tek OCT.  Thanks,

Patrick Laurie, HT
Benaroya Research Institute
1201 9th Ave
Seattle, Wa  98101
(206) 341-0681








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