Re: [Histonet] Cryosectioning mouse Eyeball


Preparing frozen sections of mouse eyeballs is difficult, especially if you 
would like to retain the morphology of the structures of interest.

We have been successful using the CryoJane Tape-Transfer system. Please 
visit the Instrumedics' web site for the details on the CryoJane method.

If you have any questions please contact us.

----- Original Message ----- 
From: "Patrick Laurie" 
Sent: Tuesday, January 17, 2006 12:36 PM
Subject: [Histonet] Cryosectioning mouse Eyeball

I have a couple of Researchers here who are interested in cryosectioning
mouse eyeballs to look at the corneas.  I have given them a few hints
(colder temperatures, cutting quickly and evenly, etc.) but they are
having variable results.  I was wondering if anyone has any further
hints.  Is there some sort of freezing media that someone would
recommend?  We are using Tissue-Tek OCT.  Thanks,

Patrick Laurie, HT
Benaroya Research Institute
1201 9th Ave
Seattle, Wa  98101
(206) 341-0681

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