>>or not use any counterstain at all since the antigenic site is so small
 
Yes, that is what I've done but they would be sooo pretty to photograph with a background stain...
 
Cheers,
Lorie
 
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-----Original Message-----
From: Gayle Callis 
To: bjdewe@aol.com
Sent: Fri, 20 Jan 2006 08:30:59 -0700
Subject: Re: [Histonet] Counter stain


Eosin? or not use any counterstain at all since the antigenic site is so small, hard to visualize after hematoxylin. 
 
At 11:28 AM 1/19/2006, you wrote: 
>I am doing a peroxidase DAB stain for an antigen that is present only in >small quantities in the nucleus. I am doing it on rat bone. If I >counterstain with hematoxylin it covers the DAB stain. What else can I use >that will only stain the cytoplasm and not the nucleus. i thought of >methyl green but it too is supposed to stain the nucleus. I thought of >neutral red but it won't look so good with the brown. Suggestions? 
> 
>Cheers, 
>Lorie 
> 
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Gayle Callis 
Research Histopathology Supervisor 
Veterinary Molecular Biology 
Montana State University - Bozeman 
PO Box 173610 
Bozeman MT 59717-3610 
406 994-6367 
406 994-4303 (FAX) 
 
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