Re: [Histonet] Counter stain

From:John Kiernan

The best contrasting colour for DAB-brown is

Ethyl green stains nuclei, so avoid it.
(Methyl green has not been made for 25+ years.
Dyes sold as "methyl green" should be ethyl green,
which is better. Check with the vendor, because
real methyl green won't perform properly without
laborious purification by you in your lab.)

For counterstaining cytoplasm but not nuclei, try
fast green FCF. Instructions can be found in any
textbook published since about 1940. Light green
SF (pre-1940) acts similarly, but fades after a
few years. 

John Kiernan
Anatomy, UWO
London, Canada
____________________________________________ wrote:
> I am doing a peroxidase DAB stain for an antigen that is present only in small quantities in the nucleus. I am doing it on rat bone. If I counterstain with hematoxylin it covers the DAB stain. What else can I use that will only stain the cytoplasm and not the nucleus. i thought of methyl green but it too is supposed to stain the nucleus. I thought of neutral red but it won't look so good with the brown. Suggestions?
> Cheers,
> Lorie
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