Yep.  I use a Santa Cruz goat poly at 1:400 with the Vector Elite HRP kit. 
 I've tried it with both HIER and without - no difference - so no HIER is 
required.
JO'C





"Greg Dobbin" 
Sent by: histonet-bounces@lists.utsouthwestern.edu
01/20/2006 12:04 PM

 
        To:     histonet@lists.utsouthwestern.edu
        cc:     djones@avcn1.novell.upei.ca, (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT)
        Subject:        [Histonet] Anti-GRP-78


Hello Everyone,

A colleague is about to stain (IHC) rat tissues (formalin fixed, paraffin 
embedded) for GRP-78. She has asked me to post her questions:

Does anyone out there have previous experience with this marker? 
And if so, do you have any tips, especially with regard to antigen 
retrieval methods (if necessary). (She doing a lit search as I write!)

Thanks so much.
Greg

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet