We use the Wolbach's Giemsa but he doesn't care for it for this purpose.  Thanks.
 

Jeanine Bartlett, HT(ASCP) 
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov 

 

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From: Rene J Buesa [mailto:rjbuesa@yahoo.com] 
Sent: Thursday, January 26, 2006 8:55 AM
To: Bartlett, Jeanine; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] technique (Gimenez?) for rickettsiae


Jeanine:
Between 1911 and 1945 there were 21 methods/variants developed for rickettsiaeThe one by Wolbach (1919) stain nuclei and Rickettsiae blue and cytoplasm red.
Lépine's (1932) stain Rickettsiae red in pale brown cells. Many others are variations of red agains blues (similar to Giemsa used for this purpose). I prefer Wolbach's.
Hope this will help.
René J.

"Bartlett, Jeanine"  wrote:

	Hi all:
	
	Can anyone recommend a good technique for demonstration of rickettsiae
	in FFPE tissue ?
	
	Thanks,
	
	Jeanine Bartlett, HT(ASCP)
	Centers for Disease Control and Prevention
	1600 Clifton Road, MS/G-32
	18/SB-114
	Atlanta, GA 30333
	(404) 639-3590 
	jeanine.bartlett@cdc.hhs.gov
	
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